Balancing the Fluorescence Imaging Budget for All-Optical Neurophysiology Experiments

Abstract The goal of this chapter is to establish a framework evaluate imaging methodologies for all-optical neurophysiology experiments. This not an exhaustive review fluorescent indicators and modalities but rather aims distill the functional principles driving choice both. Scientific priorities determine whether strategy based on “optimal indicator” or modality.” first constrains second due each’s contributions fluorescence budget signal-to-noise ratio time-varying changes.